Transcription of Drosophila mobile element gypsy (mdg4) in heat-shocked cells
نویسندگان
چکیده
منابع مشابه
Is the major Drosophila heat shock protein present in cells that have not been heat shocked?
When eukaryotic cells are exposed to elevated temperatures they respond by vigorously synthesizing a small group of proteins called the heat shock proteins. An essential element in defining the role of these proteins is determining whether they are unique to a stressed state or are also found in healthy, rapidly growing cells at normal temperatures. To date, there have been conflicting reports ...
متن کاملIn vitro transcription of a human hsp 70 heat shock gene by extracts prepared from heat-shocked and non-heat-shocked human cells.
A cDNA clone homologous to HeLa cell mRNA encoding the 70 kDa human heat shock protein (hsp 70) was used to isolate a recombinant phage containing an entire human hsp 70 gene. Sequence analysis of the 5' and 3' flanking regions revealed the structural integrity of the regulatory elements. The functional integrity of this hsp 70 gene was substantiated by in vitro transcription studies with nucle...
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The intracellular localization and expression of hsp27 (heat-shock protein 27) were investigated by cellular fractionation and immunofluorescence microscopy in Drosophila S3 cells. In unstressed cells, hsp27 is expressed in only 2% of the cells, whereas following heat shock, during recovery or after induction by ecdysone, the protein is detected in all cells. Under all these conditions, hsp27 a...
متن کاملAn Atypical Unfolded Protein Response in Heat Shocked Cells
BACKGROUND The heat shock response (HSR) and the unfolded protein response (UPR) are both activated by proteotoxic stress, although in different compartments, and share cellular resources. How these resources are allocated when both responses are active is not known. Insight in possible crosstalk will help understanding the consequences of failure of these systems in (age-related) disease. RE...
متن کاملActive gypsy / Ty 3 retrotransposons or retroviruses in Caenorhabditis elegans ( nematode / transcription / reverse transcription / mobile elements )
A gypsy/Ty3-class retrotransposon (Cerl) is integrated in the DNA of Caenorhabditis elegans chromosome III. It is 8865 nt in length and has 492-nt long terminal repeats that are identical in DNA sequence. There is an exceptionally long (6819 nt) open reading frame uninterrupted by frameshift mutations in the period since the insertion, which must therefore have been rather recent. Alignment wit...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1993
ISSN: 0014-5793
DOI: 10.1016/0014-5793(93)81079-f